منابع مشابه
Preparation of pure lipid hydroperoxides.
Increasing evidence of lipid peroxidation in food deterioration and pathophysiology of diseases have revealed the need for a pure lipid hydroperoxide (LOOH) reference as an authentic standard for quantification and as a compound for biological studies in this field. Generally, LOOH is prepared from photo- or enzymatically oxidized lipids; however, separating LOOH from other oxidation products a...
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Algal cultures have been freed of bacterial contamination by a procedure involving treatment with a detergent and phenol, followed by plating on an agar medium for selection of pure cultures.
متن کاملPreparation of pure oligonucleotide-alkaline phosphatase conjugates.
The utility and sensitivity of enzyme-labeled oligonucleotide probes have been described (1, 2). However, most methods used for their preparation require the use of extensive column chromatography, polyacrylamide gel electrophoresis, or high-pressure liquid chromatography to yield a pure product (3-5). Commercial kits offer a simple alternative but typically 1) are expensive, 2) utilize freeze ...
متن کاملThe Preparation of Relatively Pure Bacteriophage
The method described above, based on the electrophoretic migration of bacteriophage particles into an agar gel and their subsequent re-suspension in a suitable medium, has the following advantages: It is simple and can be readily carried out on a comparatively large scale by merely inserting additional units between the same electrode cups. It requires but one extraction and the resulting phage...
متن کاملOn the Preparation and Properties of Pure Glutathione (glutaminyl-cysteine)
Glutathione was first. isolated in 1921 from yeast, mammalian liver, and muscle by Hopkins (5), who also presented evidence indicating that the substance is a dipeptide of cysteine and glutamic acid. In 1925 Stewart and Tunnicliffe (8) published their work on the synthesis of glutathione. Their second synthetic method yielded a product, which possessed all of the properties of the substance fou...
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ژورنال
عنوان ژورنال: Nature
سال: 1966
ISSN: 0028-0836,1476-4687
DOI: 10.1038/211310a0